Efektivitas Anti Biofilm Ekstrak Etanol Daun Bidara Terhadap Bakteri Staphylococcus aureus
DOI:
https://doi.org/10.55606/srj-yappi.v4i2.2473Keywords:
Biofilm degradation, Phytochemical screening, Ziziphus mauritiana Lamm, Staphylococcus aureus biofilm, Antibiofilm activityAbstract
This research aims to determine the effect of ethanol extract of bidara leaves on the degradation of S. aureus biofilms. This research is an experimental study with 7 treatment groups. KN (negative control given distilled water), KP (positive control, given Erythromycin), ethanol extract concentration treatment groups namely P1 60%, P2 70%, P3 80%, P4 90%, P5 100%. The research design used was 28 samples calculated using the Federer formula. The biofilm degradation test was carried out using the crystal violet binding assay technique. Data were analyzed using the Kruskal Wallis test with a p value of 0,005 (<0,05) which was significantly different and continued with the Mann-Whitney Post Hoc test. Biofilm degradation test results of bidara leaf ethanol extract at concentrations of 60%, 70%, 80%, 90% and 100% were able to degrade S. aureus bacterial biofilms up to 19,32%, 5,42%, 10,72%, 24,16% and 38,80%, in the positive control groups (erythromycin) it was 94,84% and in the negative control (distilled water) 0,00%. It can be concluded that the ethanol extract of bidara leaves (Z. mauritiana Lamm) is able to degrade S. aureus bacterial biofilms up to 38,80%.
References
1. Becker K, Heilmann C, Peters G. Coagulase-negative Staphylococci. Clin Microbiol Rev. 2014;27(4):870-926.
2. Todar K. Todar's Online Textbook of Bacteriology. University of Wisconsin; 2020.
3. Carroll KC, Hobden JA, Miller S, et al. The Staphylococci. In: Jawetz M, & Adelberg’s, ed. Medical Microbiology. 27 ed. McGraw-Hill Education; 2019.
4. Mandal A. Staphylococcus Aureus Virulence Factors. . News-Medical. Accessed February, 15, 2023, 2023. https://www.news-medical.net/health/Staphylococcus-Aureus-Virulence-Factors.aspx.
5. Ahmad-Mansour N, Loubet P, Pouget C, et al. Staphylococcus aureus Toxins: An Update on Their Pathogenic Properties and Potential Treatments. Toxins (Basel). 2021;13(10):677.
6. Kong C, Neoh H-m, Nathan S. Targeting Staphylococcus aureus Toxins: A Potential form of Anti-Virulence Therapy. Toxins (Basel). 2016;8(3):72.
7. Tam K, Torres VJ. Staphylococcus aureus Secreted Toxins and Extracellular Enzymes. Microbiol Spectr. 2019;7(2):10.1128/microbiolspec.GPP3-0039-2018.
8. Soares B, Melo D, Motta C, et al. Characterization of virulence and antibiotic profile and agr typing of Staphylococcus aureus from milk of subclinical mastitis bovine in State of Rio de Janeiro. Arq Bras Med Vet Zootec. 2017;69:843-850.
9. Acosta AC, Oliveira PRF, Albuquerque L, et al. Frequency of Staphylococcus aureus virulence genes in milk of cows and goats with mastitis. Pesquisa Veterinária Brasileira. 2018;38:2029-2036.
10. Ganesh VK, Barbu EM, Deivanayagam CC, et al. Structural and biochemical characterization of Staphylococcus aureus clumping factor B / ligand interactions. J Biol Chem. 2011;286(29):25963-72.
11. Abrar M, Wibawan IWT, Priosoeryanto BP, Soedarwanto M, Pasaribu FH. Peranan hemaglutinin Staphylococcus aureus dalam proses adhesi pada sel epitel ambing sapi perah. J Kedokt Hewan. 2013;7(1)
12. Larasati SA, Windria S, Cahyadi AI. Kajian Pustaka: Faktor-faktor Virulensi Staphylococcus aureus yang Berperan Penting dalam Kejadian Mastitis pada Sapi Perah. Indonesia Medicus Veterinus. 2020;9(6):984-999
13. Foster TJ, Geoghegan JA, Ganesh VK, Höök M. Adhesion, invasion and evasion: the many functions of the surface proteins of Staphylococcus aureus. Nature reviews Microbiology. 2014;12(1):49-62.
14. Khusnan K, Prihtiyantoro W, Hartatik H, Slipranata M. Karakterisasi faktor-faktor virulensi Staphylococcus aureus asal susu kambing peranakan ettawa secara fenotip dan genotip. Jurnal Sain Veteriner. 2016;34(1):130-143.
15. National Centre for Disease Control. National treatment guidelines for antimicrobial use in infectious diseases. Directorate General of Health Services, Ministry of Health and Family Welfare Government of India; 2016.
16. Herrera A, Vu BG, Stach CS, et al. Staphylococcus aureus β-Toxin Mutants Are Defective in Biofilm Ligase and Sphingomyelinase Activity, and Causation of Infective Endocarditis and Sepsis. Biochemistry. 2016;55(17):2510-2517.
17. Afzal M, Vijay AK, Stapleton F, Willcox M. Virulence Genes of Staphylococcus aureus Associated With Keratitis, Conjunctivitis, and Contact Lens-Associated Inflammation. Transl Vis Sci Technol. 2022;11(7):5-5.
18. Oliveira D, Borges A, Simões M. Staphylococcus aureus Toxins and Their Molecular Activity in Infectious Diseases. Toxins (Basel). 2018;10(6):252.
19. Mardiah M. Uji Resistensi Staphylococcus aureus Terhadap Antibiotik, Amoxillin, Tetracyclin dan Propolis. Jurnal Ilmu Alam Dan Lingkungan. 2017;8(2):1-6.
20. Aisyah N, Harahap MR, Arfi F. Analisis Fitokimia dan Uji Aktivitas Antibakteri Ekstrak Daun Bidara (Ziziphus mauritiana L.) terhadap Esherichia coli dan Staphylococcus aureus. AMINA. 2021;2(3):106-113.
21. Muharrami LK, Munawaroh F, Ersam T, et al. Antibacterial Activity of Leaves Extract of Bukkol (Ziziphus mauritania Lam) against E. coli and S. aureus. KnE Engineering. 2019:180–189-180–189.
22. Hastiana Y, Handaiyani S, Agustin I. Test of Phytocemical Levels of Bidara (Ziziphus spina-christi L.) Potential as Medicinal Plants. Jurnal Mangifera Edu. 2022;6(2):182-196.
23. Dhuha NS, Haeria H, Putri HE. Toksisitas Akut Ekstrak Etanol Daun Bidara (Ziziphus spina-christi L.) berdasarkan Gambaran Morfologi dan Histologi Hati Mencit. ad-Dawaa'Journal of Pharmaceutical Sciences. 2019;2(1)
24. Ma'ruf MT, Dewi PS, Nurlitasari DF. Efficacy of Bidara Leaf (Ziziphus Mauritiana) Viscous Extract to Gingival Wound Healing in Wistar Rats. J Int Dent Medical Res. 2021;14(4):1367-1372.
25. Khoirunnisak K, Ningrum WA, Wirasti W, Rahmatullah S. Uji aktivitas antibakteri ekstrak etanol daun bidara (Ziziphus mauritiana lamm) dalam formulasi sediaan sabun cair sebagai antiseptik terhadap bakteri Stapylococcus aureus ATCC 25923. Medical Sains: Jurnal Ilmiah Kefarmasian. 2020;5(1):89-98.
26. Asy'syifa NS, Darusman F, Dewi ML. Uji Aktivitas Antibakteri Ekstrak Daun Bidara Arab (Ziziphus spina-christi L.) terhadap Staphylococcus aureus dan Escherichia coli. Prosiding Farmasi. 2020;6(2):616-620.
27. Haeria H, Dhuha N, Habra R. Aktivitas Antibakteri Fraksi-fraksi Daun Bidara (Ziziphus mauritiana). ad-Dawaa' Journal of Pharmaceutical Sciences. 2018;1(2)
28. Kurnia B, Lily S, Suharno RE. Analysis of sea bidara leaf (Ziziphus mauritiana) ethanol extract biorespon on hydrophobicity and phospholipase of Streptococcus pyogenes cell surface in tonsillitis isolate. Bali Medical Journal. 2021;10(2):572-578.
29. Akhdiya A. Quorum Sensing Bakteri: Manipulasi dan Potensi Aplikasinya Dalam Bioteknologi Pertanian. 2018.
30. Donlan RM, Costerton JW. Biofilms: Survival Mechanisms of Clinically Relevant Microorganisms. Clin Microbiol Rev. 2002;15(2):167-193.
31. Donlan RM. Biofilms: microbial life on surfaces. Emerg Infect Dis. 2002;8(9):881.
32. Moghadam SO, Pourmand MR, Aminharati F. Biofilm formation and antimicrobial resistance in methicillin-resistant Staphylococcus aureus isolated from burn patients, Iran. The Journal of Infection in Developing Countries. 2014;8(12):1511-1517.
33. Mirzaei B, Babaei R, Asiabar APD, Bameri Z. Detection of both van A & van B genes in van A phenotypes of Enterococci by Taq Man RT-PCR. Braz J Microbiol. 2015;46:161-165.
34. Purbowati R, Rianti EDD, Ama F. Kemampuan Pembentukan Slime Pada Staphylococcus epidermidis, Staphylococcus aureus, MRSA dan Escherichia coli. Jurnal Florea. 2017;4(2):1-10.
35. Ghafourian S, Mohebi R, Rezaei M, et al. Comparative analysis of biofilm development among MRSA and MSSA strains. Roum Arch Microbiol Immunol. 2012;71(4):175-82.
36. Rizki R, Des M. Teknik Pengumpulan Data Sampel Tumbuhan Untuk Pembuatan Spesimen Herbarium. Center for Open Science. 2019;November 2019:1-9.
37. Qudsiyyah F. Uji efektivitas ekstrak etanol daun Bidara (Ziziphus mauritiana) sebagai antibakteri terhadap Staphylococcus aureus dan Escherichia coli. Universitas Islam Negeri Maulana Malik Ibrahim; 2021.
38. Farnsworth NR. Biological and phytochemical screening of plants. J Pharm Sci. 1966;55(3):225-276.
39. Mauludiyah EN, Darusman F, Darma GCE. Skrining Fitokimia Senyawa Metabolit Sekunder dari Simplisia dan Ekstrak Air Daun Bidara Arab (Ziziphus spina-christi L.). Prosiding Farmasi. 2020;6(2):1084-1089.
41. Hanin NNF, Pratiwi R. Kandungan fenolik, flavonoid dan aktivitas antioksidan ekstrak daun paku laut (Acrostichum aureum L.) fertil dan steril di Kawasan Mangrove Kulon Progo, Yogyakarta. Journal of Tropical Biodiversity and Biotechnology. 2017;2(2):51.
42. Oktavia FD, Sutoyo S. Skrining fitokimia, kandungan flavonoid total, dan aktivitas antioksidan ekstrak etanol tumbuhan Selaginella doederleinii. Jurnal Kimia Riset. 2021;6(2):141.
43. Górniak I, Bartoszewski R, Króliczewski J. Comprehensive review of antimicrobial activities of plant flavonoids. Phytochem Rev. 2019;18:241-272.
44. Mbaveng AT, Hamm R, Kuete V. 19 - Harmful and Protective Effects of Terpenoids from African Medicinal Plants. In: Kuete V, ed. Toxicological Survey of African Medicinal Plants. Elsevier; 2014:557-576.
45. Guimarães AC, Meireles LM, Lemos MF, et al. Antibacterial Activity of Terpenes and Terpenoids Present in Essential Oils. Molecules. 2019;24(13):2471.
46. Farha AK, Yang Q-Q, Kim G, et al. Inhibition of multidrug-resistant foodborne Staphylococcus aureus biofilms by a natural terpenoid (+)-nootkatone and related molecular mechanism. Food Control. 2020;112:107154.
47. Gutiérrez-del-Río I, López-Ibáñez S, Magadán-Corpas P, et al. Terpenoids and polyphenols as natural antioxidant agents in food preservation. Antioxidants. 2021;10(8):1264.
48. Mukhlishoh F, Verdianti P, Krihariyani D. The resistance profile of bacteria Salmonella sp towards antibacterial ethanol extract of Bidara leaves (Ziziphus mauritiana). 2018:381-385.
49. Ekambaram SP, Perumal SS, Balakrishnan A. Scope of hydrolysable tannins as possible antimicrobial agent. Phytother Res. 2016;30(7):1035-1045.
50. Saraswati AT, Sugihartuti R, Puspitasari Y, et al. Antibacterial Activity of Bidara Leaf Extract (Ziziphus mauritiana) Against Staphylococcus aureus Isolated from Mastitis Case In Vitro. Journal of Basic Medical Veterinary. 2023;12(2):85-91.
51. Putri PA, Chatri M, Advinda L. Karakteristik saponin senyawa metabolit sekunder pada tumbuhan. Jurnal Serambi Biologi. 2023;8(2):252-256.
52. Ravi L, Manasvi V, Lakshmi BP. Antibacterial and antioxidant activity of saponin from Abutilon indicum leaves. Asian Journal of Pharmaceutical and Clinical Research. 2016;9(Suppl. 3):344-347.
53. Zhao Y, Su R, Zhang W, Yao G-L, Chen J. Antibacterial activity of tea saponin from Camellia oleifera shell by novel extraction method. Industrial Crops and Products. 2020;153:112604.
54. Parnomo T. Effect of Arabica Coffee Bean Extract (Coffea arabica) as a Growth Inhibitor of Enterococcus faecalis ATCC 29212. Journal of Drug Delivery and Therapeutics. 2021;11(3):89-96.
55. Dewi M, Darmawi D, Nurliana N, et al. Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh. Jurnal Sain Veteriner. 2020;38(1):1-6.
56. Deryabin DG, Inchagova KS. Inhibitory effect of aminoglycosides and tetracyclines on quorum sensing in Chromobacterium violaceum. Microbiology. 2018;87(1):1-8.
57. Threenesia A, Ramadhian MR. Perbandingan Efek Pemberian Ekstrak Etanol Daun Kemangi (Ocimum sanctum L.) terhadap Daya Hambat Pertumbuhan Staphylococcus aureus dan Salmonella typhi Secara In Vitro. J Agromedicine. 2019;6(1):120-124.
58. Trianes J, Bastian B, Hartati D. Differences in Diameter of the Growth Inhibition Zone of Klebsiella pneumonia Bacteria After Incubation at 37 C and 25 C. Indonesian Journal of Medical Laboratory Science and Technology. 2022;4(2):120-127.
59. Halla S, Rohmi R, Agrijanti A. Efektivitas Inkubator Portable Sebagai Alat Inovasi Penunjang Laboratorium Mikrobiologi. Jurnal Analis Medika Biosains (JAMBS). 2019;6(1):66-72.
60. Lahiri D, Dash S, Dutta R, Nag M. Elucidating the effect of anti-biofilm activity of bioactive compounds extracted from plants. J Biosci. 2019;44(2):52.
61. Hadjicharalambous A, Bournakas N, Newman H, Skynner MJ, Beswick P. Antimicrobial and Cell-Penetrating Peptides: Understanding Penetration for the Design of Novel Conjugate Antibiotics. Antibiotics (Basel, Switzerland). 2022;11(11)
62. Bhalani DV, Nutan B, Kumar A, Singh Chandel AK. Bioavailability Enhancement Techniques for Poorly Aqueous Soluble Drugs and Therapeutics. Biomedicines. 2022;10(9)
63. Andrade JC, da Silva ARP, Freitas MA, et al. Control of bacterial and fungal biofilms by natural products of Ziziphus joazeiro Mart. (Rhamnaceae). Comp Immunol Microbiol Infect Dis. 2019;65:226-233.
64. Farha AK, Yang Q-Q, Kim G, et al. Tannins as an alternative to antibiotics. Food Bioscience. 2020;38:100751.
65. Wen Q-H, Wang R, Zhao S-Q, Chen B-R, Zeng X-A. Inhibition of Biofilm Formation of Foodborne Staphylococcus aureus by the Citrus Flavonoid Naringenin. Foods. 2021;10(11):2614.
66. Lopes LAA, dos Santos Rodrigues JB, Magnani M, de Souza EL, de Siqueira-Júnior JP. Inhibitory effects of flavonoids on biofilm formation by Staphylococcus aureus that overexpresses efflux protein genes. Microb Pathog. 2017;107:193-197.
67. Sobolewska D, Galanty A, Grabowska K, Makowska-Wąs J, Wróbel-Biedrawa D, Podolak I. Saponins as cytotoxic agents: an update (2010–2018). Part I—steroidal saponins. Phytochem Rev. 2020;19(1):139-189.
68. Rosyada AG, Prihastuti CC, Sari DNI, et al. Aktivitas antibiofilm ekstrak etanol kulit bawang merah (Allium cepa L.) dalam menghambat pembentukan biofilm Staphylococcus aureus ATCC 25923. Jurnal Kedokteran Gigi Universitas Padjadjaran. 2023;35(1):34-42.
69. Mahdally NH, George RF, Kashef MT, Al-Ghobashy M, Murad FE, Attia AS. Staquorsin: A Novel Staphylococcus aureus Agr-Mediated Quorum Sensing Inhibitor Impairing Virulence in vivo Without Notable Resistance Development. Front Microbiol. 2021;12
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